Apoptosis Reagents and Kits
American Research Products Inc ONE STEP TUNEL FLOW CYTOMETRY
5000265051 ONE STEP TUNEL FLOW CYTOMETRY
ABclonal Technology Caspase-1 Rabbit pAb
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.
AdipoGen WP1130
Chemical. CAS 856243-80-6. Formula C19H18BrN3O. MW 384.3. WP1130 is a second-generation tyrphostin derivative initially identified as a JAK2 signaling inhibitor through JAK2 ubiquitination that reduces STAT activation stimulated by cytokines such as IL-6 and IL-3 through the rapid down-regulation of upstream JAK kinases. WP1130 has also been identified as a partially selective cell permeable deubiquitinating enzyme/deubiquitinase DUB inhibitor that directly inhibits DUB activity of USP9x, USP5, USP14, USP24, UCH37, UCHL1 and UCHL5, leading to rapid accumulation of polyubiquitinated K48/K63-linked proteins into juxtanuclear aggresomes and tumor cell apoptosis, accounting for its anti-cancer properties. Through this mechanism, WP1130 has been shown to downregulate the antiapoptotic proteins Bcr/Abl and Jak2 and to upregulate the proapoptotic proteins MCL-1 and p53.
Abcam Human Tau (phospho T217) Antibody Pair - BSA and Azide free, 10 x 96 Tests
Human Tau (phospho T217) Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Tau (phospho T217).
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC Annexin A4/ANXA4 Mo 50ug
Recombinant mouse Annexin A4 (ANXA4) is supplied as a lyophilized powder with N-terminal 10xHis and C-terminal Myc tags, produced in E. coli. The protein is designed for biochemical assays, antibody validation, structural studies, and investigations of membrane dynamics and exocytosis. It is provided with high purity and low endotoxin, formulated in a Tris-HCl/NaCl/trehalose buffer, and should be aliquoted and frozen for long-term storage.
- Lyophilized recombinant protein for flexible reconstitution.
- N-terminal His and C-terminal Myc tags for detection and purification.
- Greater than 90% purity by reducing SDS-PAGE.
- Low endotoxin (<1 EU/μg) suitable for sensitive assays.
- Formulated in 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
- Recommended storage at -20°C with aliquots at -80°C for extended stability.
- Available in multiple sizes, including 50 μg.
TRIBIOSCIENCE INC Caspase-1 Fluorometric Assay Kit 100 Tests
Tribioscience Caspase-1 Activity Fluorometric Assay Kit provides a simple and convenient method for assaying the activity of caspases that recognize the sequence YVAD. The assay is based on the detection of the free AFC fluorescence after cleavage from the labeled substrate YVAD-AFC. The free AFC can be quantified using a fluorometer or a fluorescence microtiter plate reader at Ex400/Em505nm. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows the determination of the fold increase in caspase-1 activity.
Medchemexpress LLC Annexin A4/ANXA4 Mo 5ug
Recombinant mouse Annexin A4 (ANXA4) fragment (residues E2-D319) supplied as a lyophilized His-Myc-tagged protein for biochemical and molecular biology research. Expressed in E. coli and purified to greater than 90% purity, the product is formulated for stability in 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0, and is intended for research use only.
- Recombinant fragment spanning residues E2-D319.
- N-terminal 10xHis and C-terminal Myc tags for detection and purification.
- Expressed in E. coli for high-yield production.
- Purified to greater than 90% purity by reducing SDS-PAGE.
- Lyophilized formulation with trehalose for enhanced stability.
- Stable when stored at -20°C; reconstituted material stable at 4°C for one week.
- Available in small research sizes, including 5 μg.
Molecular Devices Reporter Assay Kit, Firefly Luciferase, Glow, Explorer
The SpectraMax Glo Steady-Luc Reporter Assay Kit provides a sensitive, glow-type luminescence assay for quantifying firefly luciferase expression in mammalian cells. It offers extended signal stability and a wide dynamic range suitable for high-throughput microplate screening workflows.
- Sensitive quantitation of firefly luciferase in mammalian cells.
- Glow-type luminescence with extended signal stability up to 5 hours (within 20% of initial signal).
- Linear detection across approximately seven orders of magnitude.
- Explorer kit sufficient for 2 x 96-well plates; bulk option available for higher throughput.
- Optimized for luminescence microplate readers and plate reader software workflows.
- Suitable for high-throughput screening and batch plate processing.
Genscript Corporation Annexin V, His, Human
ANXA5, also known as annexin V, is a member of the annexin family of calcium-dependent phospholipid binding proteins which are involved in membrane-related activity along exocytotic and endocytotic pathways. ANXA5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel properties. It functions in signal transduction, inflammation, cell growth and differentiation. ANXA5 is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. There are at least ten different annexins in the mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.The binding of labeled ANXA5 to phosphatidylserine is commonly used as a marker of apoptosis.
Abcam Human CD64 Antibody Pair - BSA and Azide free, 10 x 96 Tests
Human CD64 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human CD64.
The product is subject to the following: Abcam Restricted Use Statement
American Research Products Inc ONE STEP TUNEL FLOW CYTOMETRY
5000265221 ONE STEP TUNEL FLOW CYTOMETRY
Sigma Aldrich Fine Chemicals Biosciences Duolink(R) In Situ Wash Buffers, Fluorescence | 4L
Duolink(R) In Situ Wash Buffers, Fluorescence | 4L